http://hdl.handle.net/10564/2233 1989-06 Fri, 10 Apr 2026 15:35:44 GMT 2026-04-10T15:35:44Z http://hdl.handle.net/10564/2261 タイトル: ヒト第Ⅹ因子の測定 ：Ⅱ. 新生児期における第Ⅹ因子活性および第Ⅹ因子抗原量について 著者: 金廣, 昭美 抄録: Factor Ⅹ activity by solid-phase immunochromometric assay (F. X : Cɪcm) and factor Ⅹ antigen by ELISA (F. X : Ag) were measured in cord plasmas from newborn infants, and in plasmas of full-term newborn infants during the first month of life without hemorrhagic symptoms. In each group, 50 plasma samples (25 males and 25 females) were collected. In the newborn cord plasma, the levels of F. X : Cɪcm (39.3±8.4 U/dl) and F. X : Ag (40.6±8.1 U/dl) were lower than normal plasma. F. X : Cɪcm and F. X : Ag in the newborn plasma were lowest on the next day of life, i. e., 33.0±9.5 U/dl and 34.6±9.3 U/dl respectively, and increased gradually during the first month, reaching 66.0±13.5 U/dl and 65.7±12.4 U/dl respectively. There was no significant difference in F. X : Cɪcm or F. X : Ag between males and females in each group. These results are discussed in connection with prothrombin, factor Ⅶ and factor Ⅸ values in the newborn period. Thu, 29 Jun 1989 15:00:00 GMT http://hdl.handle.net/10564/2261 1989-06-29T15:00:00Z http://hdl.handle.net/10564/2260 タイトル: 新生児期，乳児期におけるプロテインSの推移 著者: 橋本, 憲治 抄録: Chronological changes of levels and plasma distribution of vitamin K-dependent protein S (PS) were studied in infants throughout the first 12 months of life. Total PS antigen (T-PS : Ag), C₄b-binding protein　(C₄bp : Ag), free PS antigen (F-PS : Ag), PS : Ag complexed with C₄b-binding protein (C₄bp-S : Ag) and PS activity (PS : Act) were measured in 450 full term infants with no bleeding and thrombotic episodes. T-PS : Ag assayed by ELISA were 22.4±9.4 U/dl (mean±SD) on 0 day after birth (cord plasma), 22.3±9.4 U/dl on 1 day and rose gradually, reaching 72.0±16.8 U/dl at 12 months of age. PS : Act assayed by modified A-PTT using PS-immunodepleted plasma showed no increase but considerably higher levels in contrast with T-PS : Ag during the first 1 month of age, and then started to rise in parallel with T-PS : Ag, reachdsg 75.2±19.3 U/dl at 12 months of age. Although 54～57%　(same as the normal adult value) of PS were found to be bound to C₄bp in infants after 3 months of age, only 11.9%～34.0% of PS were bound in infants before 1 month of age because of relatively lower levels of C₄bp. Thu, 29 Jun 1989 15:00:00 GMT http://hdl.handle.net/10564/2260 1989-06-29T15:00:00Z http://hdl.handle.net/10564/2259 タイトル: Flow cytometryによる同種および自己抗ヒト血小板抗体の解析：Ⅰ. 酸処理後ホルマリン固定血小板を用いての抗体測定法の開発 著者: 水本, 保子 抄録: Flow cytometry (FCM) has recently been used to determine the epitopes of mouse monoclonal antibodies against platelets glycoproteins (GP). However, the utilization of this technique to detect the autologous anti-platelet antibody has been limited, because a high frequency of non-specific reaction has been observed in serum from healthy individuals. One reason for this has been thought to be related to the surface bound HLA antigens. To overcome this problem I have used acid treatment of platelets. The platelet pellets prepared by albumin density gradient separation were first treated with 0.123 M citric acid-Na₂HPO₄, pH3.0 for 10 min. at 0℃ followed by formalin fixation. Using these platelets, the percentage of fluorescence positive cells (%FPC) for HLA-A, B, C antigens markedly decreased from 97.0 to 21.3, whereas that for platelet surface IgG slightly decreased from 40.4 to 28.8. On the other hand, %FPCs of platelet GPⅠb and GPⅡb/Ⅲa complex remained almost unchanged, suggesting that the respective epitopes remain intact. The level of platelet bound IgG (PBIgG) of healthy adults and the multi transfused patients were determined. For healthy adults (n=10), the %FPC was 46.5±10.7 (mean±SD) before treatment, and 10.3±2.9 after treatment. Thus the %FPC after acid treatment were significantly decreased. In multi transfused patients (n=5), the %FPCs were almost unchanged before and after acid treatment, from 86.6±21.3 to 84.3±25.6. The average %FPC value in 50 healthy adults (25 males and 25 females) was 12.6±6.6 and the cut off value was 20.0. These results clearly indicate that acid treatment of platelets increases the availability and specificity for the detection of allo and autologous anti-platelet antibodies. Thu, 29 Jun 1989 15:00:00 GMT http://hdl.handle.net/10564/2259 1989-06-29T15:00:00Z http://hdl.handle.net/10564/2258 タイトル: エロソール吸入シンチグラフィにおける沈着パターンの検討：第二報 SPECT像とCT像の対比によるhot spot形成機序の解析 著者: 渡辺, 裕之 抄録: The superimposed images obtained by the SPECT of aerosol inhalation scintigraphy and chest CT were applied in 7 cases of diffuse panbronchiolitis. Aerosol deposition patterns were examined, and hot spots were compared with bronchial morphological abnormalities. The results were as follows : 1. Nevertheless, aerosol deposition patterns were characterized by defects of the depositions in the outer zone and hot spots in the inner zone, hot spots distributed from the inner zone to the outer zone. 2. Hot spots and bronchial morphological abnormalities were markedly matched in the inner zone ; however, they were mismatched in the outer zone. I concluded that the mechanisms of hot spot formation in the inner zone were different from those in the outer zone. Thu, 29 Jun 1989 15:00:00 GMT http://hdl.handle.net/10564/2258 1989-06-29T15:00:00Z