http://hdl.handle.net/10564/1934 1991-12 Fri, 10 Apr 2026 15:37:32 GMT 2026-04-10T15:37:32Z http://hdl.handle.net/10564/1946 タイトル: 第112回奈良医学会 : 学会記事 Mon, 30 Dec 1991 15:00:00 GMT http://hdl.handle.net/10564/1946 1991-12-30T15:00:00Z http://hdl.handle.net/10564/1945 タイトル: 抗第Ⅷ因子活性を有する数種抗第Ⅷ因子モノクローナル抗体を用いた血友病A患者血漿中の第Ⅷ因子抗原量の測定 著者: 中井, 寛明 抄録: Six monoclonal antibodies (MoAbs), NMC-Ⅷ/5-10, against factor Ⅷ (F. Ⅷ) were developed and two-site solid phase enzyme-linked immunosorbent assays (ELISAs) for factor Ⅷ antigen (F. Ⅷ : Ag) were performed using three anti-F. Ⅷ : Ag monoclonal antibodies, NMC-Ⅷ/5, Ⅷ/10 and C5 (mono-ELISAs). In these ELISAs, polyclonal anti-F. Ⅷ alloantibody (PolyAb) was used as a coating antibody and the peroxidase-conjugated MoAb as the second antibody. MoAbs NMC-Ⅷ/5-10 reacted with 80 kDa light chain and NMC-Ⅷ/5 and 6 reacted with 72 kDa light chain fragment by immunoblotting. The IgG subclass of these MoAbs was all IgG_1 and anti-F. Ⅷ inhibitory activity ranged from 2 to 160 Bethesda U/mg. C5 recognized the 54 kDa heavy chain fragment. The levels of F. Ⅷ : Ag in 20 normal individuals were 93±21 u/dl by Ⅷ-5-ELISA, 91±18 u/dl by Ⅷ-10-ELISA, 91±21 u/dl by C5-ELISA and 97±23 u/dl by the double polyclonal ELISA (poly-ELISA). Forty-nine cases with hemophilia A were divided into three groups with F. Ⅷ : Ag level by poly-ELISA ; Group 1 (<1 u/dl), Group 2 (1-49 u/dl), Group 3 (≧50 u/dl). F. Ⅷ : Ag was not detected by any mono-ELISA in 30 patients of Group 1. These patients were considered to lack F. Ⅷ protein. In 9 cases out of 13 in Group 2, the level of F. Ⅷ : C and F. Ⅷ : Ag correlated well (Group 2-A). The other 4 cases had higher level of F. Ⅷ : Ag than F. Ⅷ : C (Group 2-B). These patients were considered to have abnormal F. Ⅷ protein. Two cases out of 6 patients in Group 3 had extremely lower level of F. Ⅷ : Ag by C5-ELISA than of levels measured by other mono-ELISAs and poly-ELISA. These patients seem to have a molecular defect at or near the binding region of C5. Mon, 30 Dec 1991 15:00:00 GMT http://hdl.handle.net/10564/1945 1991-12-30T15:00:00Z http://hdl.handle.net/10564/1944 タイトル: 維持透析患者における血管内皮傷害に関する研究 ： 凝固・線溶能およびトロンボモジュリンからの検討 著者: 大野, 安男 抄録: This study was performed to evaluate the degree of vascular endothelial damage by examining coagulation activity, fibrinolytic activity and vascular endothelial cell function in patients undergoing maintenance hemodialysis treatment (MDT). Twenty-seven patients with chronic renal failure due to primary glomerular disease were enrolled in this study. The items measured were fibrinogen, antithrombin-Ⅲ (ATⅢ), thrombinantithrombin Ⅲ complex (TAT) as parameters for coagulation activity, plasminogen (Plg), α₂-plasmin inhibitor (α₂-PI), α₂-plasmin inhibitor-plasmin complex (PIC), D-dimer, plasminogen activator inhibitor-1 (PAI-1), tissue plasminogen activator (t-PA) antigen and its activity for fibrinolytic activity, and von Willebrand factor antigen (vWF : Ag) and thrombomodulin (TM) for vascular endothelial cell function. Predialysis levels of fibrinogen, TAT, PIC, D-dimer, vWF : Ag and TM in MDT patients increased significantly compared with those in normal subjects, while ATⅢ, Plg,　α₂PI, PAI-1 and t-PA antigen decreased signigicantly. During single hemodialysis, TAT, PIC, D-dimer, t-PA antigen and its activity, vWF : Ag and TM increased significantly, while PAI-1 apparently decreased. In addition, t-PA activity was consistently elevated during single hemodialysis. There was a positive correlation between predialysis level of TM and duration of MDT. These results suggest that TAT, PIC and D-dimer can be used as good indicators in the coagulation-fibrinolysis system function even in patients with impaired renal function, and that TM is also an excellent indicator in finding out the condition of vascular endothelial injury. Meanwhile, it seemed quite plausible that long-term MDT facilitates the thromboembolic lesion and the atherosclerosis in MDT patients. Mon, 30 Dec 1991 15:00:00 GMT http://hdl.handle.net/10564/1944 1991-12-30T15:00:00Z http://hdl.handle.net/10564/1943 タイトル: 実験的ラット肝蛭症の免疫診断学的および病理学的研究 著者: 吉岡, 豊 抄録: The aim of this study was to establish an experimental model for fascioliasis in rats and to investigate the relationship between immunochemical changes in the blood and histopathological changes in the liver. Experimental infection with Fasciola sp. was established in Wistar rats of 5～9 weeks old. Direct evidence of infection was obtained from ovum positive stools, which appeared at 8 weeks after infection. The findings suggested that the parasite had matured to the adult stage in the liver. Additional evidence included an increase in the levels of GOT and GPT at 3 to 8 weeks and similar increases in LAP activity at 4 to 8 weeks after infection. The raised levels of GOT and GPT reflected liver parenchyma damage caused by fluke infestation and elavated LAP provided evidence of fluke migration into the bile ducts. Marked increases in specific antibody levels were detected by ELISA as early as 1 week after infection, and specific immunoprecipitation bands were detected 2 weeks after infection. Antibody levels reached maximum at 8～10 weeks after　infection. These results suggest that the ELISA method could be especially useful in the early diagnosis of experimental fascioliasis in rats. Direct histopathological analysis of livers at intervals after infection demonstrated that : 1) metacercariae of Fasciola sp. mature to the adult stage in rats ; 2)　invasion into the liver by the parasite occurred between 2 and 4 weeks ; 3) migration to the main bile duct occurred between 6 and 8 weeks ; 4) pathological changes in the liver caused by Fasciola sp. in this model were similar to those described for human fascioliasis. In conclusion, the present studies have established a useful model for experimental infection with Fasciola sp. in 5～9-week-old rats. In addition a specific micro ELISA for antibody to Fasciola sp. has been developed for the early diagnosis of fascioliasis. The process of invasion into the liver and migration into the biliary tract was monitored by the measurement of serum enzyme activities release from hepato-biliary foci. Mon, 30 Dec 1991 15:00:00 GMT http://hdl.handle.net/10564/1943 1991-12-30T15:00:00Z