http://hdl.handle.net/10564/1512 1996-10 Fri, 10 Apr 2026 15:42:40 GMT 2026-04-10T15:42:40Z http://hdl.handle.net/10564/757 タイトル: ラットにおけるコリン欠乏アミノ酸食により誘発された肝結節性肥大と肝細胞癌におけるテロメラーゼ活性 著者: 長谷川, 清彦 抄録: Telomerase activities in hyperplastic nodules and hepatocellular car- cinomas (HCCs) induced by a choline deficient L-amino acid defined (CDAA) diet were examined by TRAP assay in rats. Male F 334 rats, 6 weeks old, received a CDAA diet for 10, 50 or 75 weeks. Hyperplastic nodules and HCCs were noted in rats fed a CDAA diet for 50 and 75 weeks, respectively. As a control, normal livers from 6 week old rats were obtained. Normal liver and background parenchyma of rats fed either of the diets for 10 weeks or 50 weeks showed weak telomerase activities. In contrast, markedly increased telomerase activities were found in hyperplastic nodules and HCCs. These results suggest that increased telomerase activity may be one biological phenomenon of preneoplastic lesions for evolution to HCCs in rat liver. Wed, 30 Oct 1996 15:00:00 GMT http://hdl.handle.net/10564/757 1996-10-30T15:00:00Z http://hdl.handle.net/10564/756 タイトル: 奈良県中和地区の救急搬送状況 著者: 今西, 正巳; 宮本, 誠司; 小延, 俊文; 野阪, 善雅; 西村, 章; 植田, 史朗; 曽我, 美純子; 三野, 浩也; 土肥, 直文; 中村, 達也; 稲田, 有史; 西尾, 健治; 村尾, 佳則 抄録: Our Department of Emergency and Critical Care Medicine in Nara Medical University opened in May, 1990. We evaluate and summarize current emergency patient transportation status and trends in the Chuwa district of Nara Prefecture on the basis of emergency transportation reports from several Emergency Medical Service (EMS) in this area. The number of outpatients in our department has decreased gradually. However, the ratio of severe patients has increased. The number of patients who visited the weekend- clinic and secondary emergency hospitals increased in Kashihara city. The number of emergency patients transported has been increasing according to the reports of EMS in the Chuwa district. However, the indistrict ratio (the ratio of patients transported to facilities inside the district which each EMS is responsible for) varied for the different EMS. The indistrict ratio increased in Chuwa and Nishikikatsuragi EMS, whereas it decreased in Sakurai and Kashiba-Koryo EMS. The time of patient transport tended to increase year after year in all EMS with the exception of Nishikatsuragi EMS. Although the in-district ratio increased, the time of patient transport tended to increase in the Chuwa district. The elongation of the addressing period to the receiving hospital, in addition to the increased time for emergency staff to reach the scene are considered to account for this situation. The increased time of patient transport in Sakurai and Kashiba -Koryo EMS was considered to be attributable to the decrease in the in-district ratio. An increase in the in-district ratio and a decrease in patient transport time were observed in Nishikatsuragi EMS every year, which was considered a model district for emergancy medicine. Wed, 30 Oct 1996 15:00:00 GMT http://hdl.handle.net/10564/756 1996-10-30T15:00:00Z http://hdl.handle.net/10564/755 タイトル: プレキャストGradient Gelを用いた非RI PCR-SSCP法によるp53遺伝子変異の検出 著者: 中林, 仁美; 岡本, 康幸; 中野, 博; 丹羽, 欣正; 久保田, 力 抄録: We optimized the convenient nonradioactive polymerase chain reaction (PCR)-based single strand conformation polymorphism (SSCP) analysis using commer- cially available precast gradient polyacrylamide gels to detect point mutations in the p53 gene. The optimizatiOn was made by using 2-15% gradient gel whose running conditions were 2×Tris glycine or 1×TBE buffer, and 200 V for 2 hours at 4℃. Also, we screened 47 patients with various hematologic malignancies and detected p53 mutations in two patients with acute leukemia. Both of them showed a resistance to chemotherapy, whereas 20 Other patients with acute leukemia and without detectable mutation did not show such a resistance except for one single case. Mutations in the p53 gene could be closely associated with a resistance to chemotherapy in leukemias. Wed, 30 Oct 1996 15:00:00 GMT http://hdl.handle.net/10564/755 1996-10-30T15:00:00Z http://hdl.handle.net/10564/754 タイトル: CLINICAL CHARACTERISTICS OF ISCHEMIC STROKE IN PATIENTS WITH DIABETES MELLITUS 著者: Kanauchi, Masao; Kawano, Takahiro; Kikawa, Tadatoshi; Akai, Mayumi; Dohi, Kazuhiro 抄録: To investigate the clinical characteristics of ischemic stroke and its associa- tions with hypertension, obesity, hyperlipidemia, and diabetic microangiopathy in patients with diabetes mellitus, we estimated the average period between onset of diabetes and ischemic stroke, and the incidences of complications. A total of 544 patients with diabetes mellitus who were admitted to our hospital over the past 10 years were analyzed, forty of whom were diabetic patients who suffered ischemic stroke as diagnosed by clinical exami- nation. As controles, 40 subjects matched for sex, age, and duration of diabetes were identified, one for each patient with ischemic stroke. The average period between onset of diabetes and ischemic stroke was significantly shorter for patients with hypertension than for normotensive patients ; however, there were no differences in the length of this period between the groups with and without obesity or hyperlipidemia. Hypertension was present in 63% of patients with ischemic stroke, and the incidence of hypertension was significantly higher in the stroke group than in the control group. There was no difference between the groups with and without ischemic stroke with respect to the incidence of obesity, hyper- lipidemia, retinopathy and nephropathy. We conclude that diabetic patients with hyperten- sion appear to be at high risk for ischemic stroke. Wed, 30 Oct 1996 15:00:00 GMT http://hdl.handle.net/10564/754 1996-10-30T15:00:00Z