http://hdl.handle.net/10564/1858 1992-10 2026-04-10T15:40:14Z http://hdl.handle.net/10564/1873 タイトル: 旋毛虫の成虫抗原に関する研究 著者: 鈴木, 秀和 抄録: A zoonotic nematoda, Trichinella spiralis, has three distinct developmental stages including muscle larva, adult, and newborn larva. The aim of this study was to define antigenic substances in adult worms of the parasite, and to assess specificity of the antigens by means of immunoelectron microscopy, which would be essential not only for developing better immunodiagnostic antigen but also for understanding of the basic issue of protective immunity. The methods included : 1) preparation of adult worms for ultrathin sections ; 2) immunostaining with sera from patients with trichinosis to localize antigenic substances in adult worms ; 3) immunostaining with sera from other parasite infections to assess specificity of the antigens ; and 4) observation under an electron microscope. Control experiments were performed to confirm specificity by immunostaining. Subcellular structures that were positive by immunostaining with patients' sera included hypodermis, hemolymph, hypodermal gland, brush border, intersperm substances, exocrine granules of male genital tract, microvilli of ova and fetus cytoplasm. cuticle inner layers were weakly positive. The surface of cuticle and stichocyte granules were negative by immunostaining. The class of the corresponding immunoglobulin was predominantly G. These adult antigens were devoid of prominent species specificity because the above-mentioned structures were more or less immunostained by sera from other helminthiasis patients including paragonimiasis, cysticercosis, fascioliasis, trichuriasis, dirofilariasis and anisakiasis. 1992-10-30T15:00:00Z http://hdl.handle.net/10564/1872 タイトル: 大和高原三村における住民健診受診者の血圧値および血清脂質の推移 ： 1987年度から1991年度の連続受診者649名の解析 著者: 榎本, 康博; 車谷, 典男; 大門, 位守; 坂梨, 照子; 和田, 昭治; 森山, 忠重; 川口, 忠男; 杉本, 和夫 抄録: Chronological changes in blood pressure and serum lipid levels were investigated in the people (232 men and 417 women) participating in annual community-based mass examinations which were carried out from 1987 to 1991 in three villages in the northern part of Nara Prefecture. The results obtained are summarized as follows ; 1) Around 40% of the people participating in the mass examinations in each year attended the examinations annually from 1987 to 1991. 2) Fourteen percent of men participating in the examinations annually had medical treatment for some kind of disease, of which 47% was hypertension. The corresponding figures for women were 16% and 70% respectively. 3) Prevalence of people judged to be hypertensive at the time of mass examinations decreased chronologically from 1987 to 1991. 4) Prevalence of the hypertensive people including under treatment for hypertension decreased chronologically in men, but increased in women. Prevalence of women with good controlled blood pressure increased chronologically. Among the hypertensives, prevalence of the persons without any treatment was the highest among men of their 50s. 5) Mean serum total cholesterol levels of both men and women were significantly higher at the last examination than the first one. 6) Serum HDL cholesterol levels showed no significant chronological change. 1992-10-30T15:00:00Z http://hdl.handle.net/10564/1869 タイトル: 血友病Aインヒビター患者におけるブタ第Ⅷ因子製剤(Hyate : C)の反応性 著者: 寺田, 茂紀 抄録: Human and porcine factor Ⅷ inhibitor titers were measured in the plasma of 15 hemophilia A patients with inhibitor and binding fragment to human factor Ⅷ polypeptide was analyzed by immunoblot method. 1. Human factor Ⅷ inhibitor levels were 4-390 B.U/ml, and porcine factor Ⅷ inhibitor levels that were 0-58 B.U/ml were lower than inhibitor levels to human factor Ⅷ. Cross reactivity (ratio of porcine to human factor Ⅷ inhibitor titer) was 0-100%. 2. Two cases with factor Ⅷ inhibitor binding to 44 kDa fragment of human factor Ⅷ heavy chain had low crossreactivity (0.5%-0%). One case with inhibitor binding to 44 kDa fragment and 72 kDa fragment of human factor Ⅷ light chain had crossreactivity of 11%. 10 cases with inhibitor binding to 72 kDa fragment had high crossreactivity (24-100%). Two cases with inhibitor binding to 44 kDa and B domain had crossreactivity of 1% and 27%. 3. Hemostatic effect of the porcine factor Ⅷ preparation was observed in 3 cases with factor Ⅷ inhibitor. 1992-10-30T15:00:00Z http://hdl.handle.net/10564/1868 タイトル: 大和高原三村の食生活構造と健診情報との相関性の検討 著者: 大門, 位守; 榎本, 康博; 車谷, 典男; 坂梨, 照子; 和田, 昭治; 森山, 忠重; 川口, 忠男; 杉本, 和夫 抄録: To clarify the characteristics of eating habits among the residents of Yamato-Kogen district we conducted a principal component analysis of food frequency questionnaire (consisting of 14 items) obtained from the people participating in community-based mass examinations. The examinations were performed in the district under study between July and September in 1991. Eigen values, contribution rations and loading factors were calculated from correlation matrix among 14 items. As the first principal component, the items of Q10 (food cooked with oil), Q7 (boiled fish pastes), Q5 (fishes), Q3 (eggs), Q13 (tsukudani), Q4 (meats), Q11 (miso soup) were extracted, and as the second one those of Q1 (cooked paddy rice), Q13 (tsukudani) in Q6 (salted, semidried, split fishes) and Q12 (tsukemono) were extracted. The first principal component was considered to represent a shift from conventinal foods in the countryside, and the second one conventional village-type foods. Principal scores of the 1st to the 3rd principal components were yielded indivisually using loading factors for each subgroup classified by sex and village. Distribution patterns of two of these components indicated that the differences were found in eating habits not only between men and women but also among the villages under study. The distribution patterns could also clearly identify the persons whose eating habits deviated nutritionally. For the same subjects a multiple regression analysis was done on all kinds of laboratory data and 14 items of food frequency questionnaire. The results revealed the existence of associations between eating habits and laboratory data. 1992-10-30T15:00:00Z