http://hdl.handle.net/10564/1615 1994-10 2026-04-10T15:40:10Z http://hdl.handle.net/10564/1633 タイトル: PROPHYLACTIC ENDOSCOPIC INJECTION SCLEROTHERAPY FOR GASTRIC VARICES : 1. DEVELOPMENT OF A NEW SCLEROTHERAPY TECHNIQUE AND ITS APPLICATION 著者: Matsumura, Masahiko 抄録: The author designed a direct injection method of endoscopic injection sclerotherapy (EIS) for gastric varices with a newly developed technique for controlling bleeding from the punctured site, and subsequently used it for prophylactic treatment in 10 cases. EIS was performed under X-ray monitoring in the absence of a balloon, and 5% ethanolamine oleate containing 49% Iopamidol was used as the sclerosant. A twenty-five gauge needle wearing an outer tube was used for the puncture. After injection of sclerosant, thrombin was poured into the outer tube while pulling out the needle. After the needle was pulled out, thrombin was sprayed over the punctured site when bleeding was negligible, and if bleeding was still noted, 1-2 ml of ethanol was injected into the varices. Bleeding from the punctured site was successfully controlled by spraying thrombin or ethanol injection. No other serious complications were observed. All varices treated by this technique were flattened or had disappeared after EIS, and since then no recurrent growth or bleeding has been observed. It is suggested that this direct injection method of EIS is a safe, less invasive and effective prophylactic treatment for gastric varices. 1994-10-30T15:00:00Z http://hdl.handle.net/10564/1632 タイトル: ラットを用いた高周波熱凝固法によるNeuropathic Pain Modelに関する研究 著者: 横山, 忠司 抄録: Background : The mechanisms of neuropathic pain are not clearly understood which are characterized by various different kinds abnormal sensation ; hence the author attempted to elucidate a novel pain model that is produced by radiofrequency neurolysis in rats. Methods : Bilateral sciatic nerves of 30 wister rats (7-8 weeks) were exposed and blocked with heat (neurolysis) under pentobarbital anesthesia. Right sciatic nerves were treated at 50℃ for 45 sec. (N=10), 60℃ for 45 sec. (N=10), 70℃ for 45 sec. (N=10) respectively ; left sciatic nerves were not treated as a sham. Rats were placed in plastic cages for behavioral testing ; posture of neurolyzed extremity, responses to spontaneous nociceptive pain and radiant heat impinged on plantar surface of hind paw. To examine neuropathologic change in sciatic nerves that were harvested after neurolysis, light microscopic sections were stained with toluidine blue. Results : Average autotomy scores were remarked during nerve reconstruction in 60℃ and 70℃ neurolysis but no significant difference was found between sham and operated paws in 50℃neurolysis. In micrographs of nerves removed 7 days after neurolysis, endoneural edema was prominent and the destruction of myelinated fibers could be seen. Conclusion : It was demonstrated that this model of neuropathic pain with radiofrequency neurolysis revealed partially similar, partially different results compared with previous models, and suggested that an optimal radiofrequency neurolysis in rats was 60℃ for 45 sec. that developed in recovery course of nerve injury. 1994-10-30T15:00:00Z http://hdl.handle.net/10564/1631 タイトル: 旋毛虫症に特異的な免疫診断法に関する研究 著者: 竹上, 貴子 抄録: Trichinella spiralis muscle larvae possessed both "specific" antigens in stichocyte α-granules, the alimentary tract, and the cuticle surface ; and "non-specific" antigens in cuticle inner layer(s), hypodermis, and hemolymph. Thin study was designed to determine immunocytochemically the class of antibodies raised against specific and non-specific antigens. Ultrathin sections of resin embedded T. spiralis muscle larvae were immunostained with sera from patients with a wide variety of helminthiasis including trichinosis, paragonimiasis, fascioliasis, trichuriasis, gnathostomiasis, dirofilariasis, anisakiasis, cysticercosis cellulosae hominis, and diphyllobothriasis. Sera from all patients reacted positively against the non-specific antigens. IgG-class antibodies exclusively contributed to the response. On the other hand, patients with trichinosis evoked both IgG- and IgM-class antibodies against specific and non-specific antigens. These results increase the potential usefulness of the specific diagnosis of trichinosis, because they suggest that a test using only IgM-class antibodies may minimize the undesirable effects of contaminating non-specific antigens in the immunodiagnosis of trichinosis. 1994-10-30T15:00:00Z http://hdl.handle.net/10564/1630 タイトル: THE EXPRESSION OF HLA-G ANTIGEN IN DIFFERENT HUMAN TISSUES AND PLACENTAS AT DIFFERENT STAGES OF PREGNANCY 著者: Hirano, Yoshinari; Ishitani, Akiko; Ito, Nobuaki; Geraghty, Daniel E. 抄録: The distribution of HLA-G antigens in human tissues including placents was examined by immunohistochemical methods, using monoclonal antibody (Mab) specific to HLA-G antigen (87 G). Comparatively, class Ⅰ specific Mab, W 6/32 was also employed for the detection of the antigen. W 6/32 reacted with a wide variety of cells and tissues throughout the body, whereas Mab 87 G did not react at all with these W 6/32 reactive cells except for those of placental tissues. Mab 87 G reacted with extravillous cytotrophoblast cells such as cytotrophoblastic cell columns, cytotrophoblastic shell and invasive cytotrophoblast but not other parts of placentas throughout the pregnant periods. Essentially the same results were obtained with W 6/32 staining in placentas. These results indicate that HLA-G antigens are exclusively expressed in extravillous cytotrophoblast cells although we cannot rule out the possibility that undetectable amounts of the HLA-G antigen or its isoforms which cannot be recognized by the Mabs used in this study are synthesized in some other tissues. 1994-10-30T15:00:00Z