1993-06 http://hdl.handle.net/10564/1798 2026-04-09T03:45:07Z 2026-04-09T03:45:07Z 安井, 潔 http://hdl.handle.net/10564/1822 2017-05-29T06:07:46Z 1993-06-29T15:00:00Z

タイトル: ネズミチフス症における免疫成立機序に関する研究 著者: 安井, 潔 抄録: Two outbred mouse strains, ddY and CF1, were tested for their ability to be protected against infection with Salmonella typhimurium by several types of salmonella vaccines. These strains have the same levels of innate susceptibility to S. typhimurium, and also have the same capacity to develop delayed-type hypersensitivity (DTH) to salmonella antigens. Both the crude ribosomal fraction (CRF) and live-cell vaccines conferred acquired resistance on both strains, characterized by greater responses of T cells to salmonella antigens. Mice of the ddY strain were also protected by the purified transfer RNA (tRNA) vaccine, which was free of O antigens, but CF1 mice were not, despite the presence of T-cell reactivity with salmonella antigens. Neither strain was protected by phenol-water-extracted lipopolysaccharide (LPS). The tRNA-immunized CF1 mice were protected by transfer of antiserum to CRF, but not by transfer of anti-LPS antibody. This antiserum to CRF, however, did not transfer acquired resistance into non-immune mice of either strain. These observations suggest that CF1 mice may require an antibody to another non-O antigen existing in CRF to develop acquired resistance, and that stimulation of the defense system by tRNA may be essential to the development of acquired resistance in CF1 mice. The active component in CRF of Salmonella typhimurium, capable of inducing protective antibody, was partially purified by two series of chromatography (Sephadex G-150 and DEAE-Sepharose CL 6B) after sodium dodecyl sulphate (SDS)-treated CRF was precipitated with ammonium sulphate. The major active component was eluted by 0.4-0.45 M Nacl from DEAE-Sepharose CL 6B, and its molecular weight was 43,000 as determined by SDS-polyacrylamide gel electrophoresis. Immunization with the fraction containing 43,000 component alone did not always confer protection on CF1 mice, but its administration together with either the purified tRNA or Freund's complate adjuvant (FCS) was much more effective against infection with S. typhimurium. Western blot analysis showed that 43,000 component did not react to ansiserum to LPS, but to antiserum to CRF. The antibody elicited by non-O antigenic component and the cell-mediated resistance stimulated by the adjuvant effect of RNA together confer effective protection on CF1 mice.

1993-06-29T15:00:00Z Okamoto, Shingo Kamata, Osamu Matsumoto, Masami Izumi, Yukiko Nishiyama, Toshimasa Fukui, Hiroshi Tsujii, Tadasu Sakaki, Toshisuke http://hdl.handle.net/10564/1814 2017-06-11T23:20:26Z 1993-06-29T15:00:00Z

タイトル: A CASE OF DIABETES INSIPIDUS ACCOMPANYING THIRST DISORDER ASSOCIATED WITH HYPEROSMOLAR DIABETIC COMA 著者: Okamoto, Shingo; Kamata, Osamu; Matsumoto, Masami; Izumi, Yukiko; Nishiyama, Toshimasa; Fukui, Hiroshi; Tsujii, Tadasu; Sakaki, Toshisuke 抄録: The case of a 14-year-old female who suffered from hyperosmolar diabetic coma (HODC) after resection of craniopharyngioma and during treatment for hypopituitarism and diabetes insipidus is presented. In Aug. 1989, craniopharyngioma was diagnosed and she underwent resection surgery and radiotherapy. Since then, she had been on supplemental therapy with hydrocortisone and thyroxin and desmopressin (DDAVP). On Jan. 17, 1992, she fell into HODC upon ingesting a large amount of soft drink to supplement water due to persistent polyuria. She improved quickly when supplementary fluids and insulin were administered. She had demonstrated no abnormality in glucose tolerance prior to this manifestation. Insulin therapy was deemed unnecessary after her recovery from HODC. Because of a disorder in the central nervous thirst mechaninm, she lacked the sense of thirst and concomitantly the thirst-mediated water intake in spite of elevated plasma osmolarity due to dehydration and hyperglycemia. This seemed to be the cause of her accelerating dehydration. The resulting insulin resistance then brought about her HODC. Thus, it is difficult to consider such a case of HODC as symptomatic of diabetes when no abnormality in glucose tolerance either before manifestation or after restoration can be found. It should rather be considered as a case of “dehydration hyperglycemia" and be treated as such.

1993-06-29T15:00:00Z 山田, 正和 http://hdl.handle.net/10564/1813 2017-05-29T06:07:46Z 1993-06-29T15:00:00Z

タイトル: 四国出土頭骨の古人類学的研究 著者: 山田, 正和 抄録: Skulls excavated in Shikoku were studied craniometrically and arranged by their localities in Century order from the Jomon to the historic period. There were 13 individuals of Tokushima, 2 of Kagawa and 16 of Ehime. Half of them were reconstructed and their physical characteristics were effectively compared by indices such as cranial index, upper-facial indices, nasal index and upper alveolar arch typing. The results were compared with those of modern skulls of the Kinki district. The Tokushima's skulls were most dolichocephalic and the others were mesocephalic. The upper faces were mostly low and wide, and the nasal index showed generally a wide type. Local differences and varieties in relating to the era were found even in Shikoku. The characteristics seem very different from those of the modern skulls of the Kinki district. From the present craniometrical basis of differences in physical characteristics, the Shikoku ancestors appear to be more independent of neighbouring immigrants and slower to mix with immigrants than the inhabitants of the Kinki district.

1993-06-29T15:00:00Z 西井, 清治 http://hdl.handle.net/10564/1803 2017-05-29T06:07:44Z 1993-06-29T15:00:00Z

タイトル: ラット腎腫瘍発生におけるリン酸塩の促進作用並びにクエン酸カリウムの抑制効果に関する研究 著者: 西井, 清治 抄録: Medium organ bioassays to shorten the experimental duration with unilateral nephrectomy for early detection of renal tumors were investigated in male Wistar rats after N-ethyl-N-hydroxyethylnitrosamine (EHEN) administration. Animals were fed 1000 ppm EHEN diet for 2 weeks and the left kidney was removed at week 3. They received several promoting agents and non-promoting agents as negative control for 18 weeks. Within a relatively short period of 20 weeks, the promoting effect on rat renal tumorigenesis can be detected as a significant increase of preneoplastic lesions, such as simple or adenomatous hyperplasias with increased levels of 5-bromo-2'-deoxyuridine (BrdU)-labeled renal cortical tubular epithelia. Various kinds of salts and metals contained in the diet were tested at concentrations more than 20 times the normal doses in a preliminary experiment for nephrotoxicity over 8 weeks. Potassium dibasic phosphate (PDP), potassium aluminum sulfate (PAS) and copper sulfate (CS) induced nephropathy. Therefore, these three salts were investigated in this medium-term bioassay for renal tumorigenesis. Treatment with 5% PDP induced renal calcification with severe nephropathy and promoted the development of preneoplastic lesions, but that with 5% PAS or 0.5% CS did not. To study the effect of alkalization on renal mineralization，animals concomitantly received 5% potassium citrate (PC). The addition of PC to PDP diet reduced the promoting effect on renal tumorigenesis ; also indicated was retardation of renal calcium by histology, serum biochemistry and urinalysis. The promoting effects of PDP and inhibitory effects of PC were correlated to BrdU-labeling indices. Immunohistochemical study was used to examine α₂ᵤ-globulin accumulation ; however, PDP-induced nephropathy did not appear to be related to α₂ᵤ globlin, as evidenced by the negative results. A pathogenesis for renal carcinogenesis is suggested in which nephropathy associated with mineralization enhanced the development of renal cell tumors.

1993-06-29T15:00:00Z