1994-12 http://hdl.handle.net/10564/1594 2026-04-10T15:42:23Z 2026-04-10T15:42:23Z http://hdl.handle.net/10564/1614 2017-05-29T06:07:32Z 1994-12-30T15:00:00Z

タイトル: 第115回奈良医学会 : 学会記事

1994-12-30T15:00:00Z 森本, 佳成 http://hdl.handle.net/10564/1613 2017-06-11T23:20:26Z 1994-12-30T15:00:00Z

タイトル: 口腔領域における交感，感覚および運動神経支配に関する研究 ： Ⅱ. 顎下腺，舌下腺，耳下腺における神経支配 著者: 森本, 佳成 抄録: Sympathetic and sensory innervation of the submandibular，sublingual and parotid glands，and difference of sensory innervation between the body and duct were studied by horseradish peroxidase (HRP) method. Numerous labeled cells were observed in the ipsilateral superior cervical (SCG) and a few in stellete ganglion (SG) after HRP injection into the submandibular and parotid gland bodies. They were localized at the central 1/2 of SCG. No labeled cells were found in the sympathetic ganglia following sublingual HRP injection. HRP injection into the submandibular，sublingual and parotid gland body brought labeling of small numbers of neurons (1.4，0.6 and 2.2，respectively) in the unilateral trigeminal ganglion (TRG). However，more TRG neurons were labeled after HRP application in the submandibular (7.8±3.4 units) and parotid duct (9.4±3.7 units) than in the gland body. Mean diameter of labeled cells from the duct was significantly smaller (18.6±3.2μm and 18.2±3.4μm，respectively) than that of tooth plup (27.4±5.0μm) or TMJ (23.7±5.9μm). No labeled neurons in SCG were recognized by submandibular gland HRP application after sectioning the facial artery or by parotid HRP injection after cutting the external carotid artery. Most of the sympathetic components supplying the submandibular or parotid gland originate from SCG and much more limitedly from SG. No sympathetic innervation exists in the sublingual gland. While the sensory innervation of the submandibular and parotid gland bodies is rare，that of their ducts is more dense by C-fibers which may generate a pain sensation related to the intraductal sialolithiasis. The sympathetic nerve from SCG and SG is supposed to innervate the submandibular or parotid gland via the feeding arteries after arising from the external carotid plexus. They do not pass by way of the lingual nerve or submandibular ganglion for the submandibular gland，or of the auriculotemporal nerve for the parotid gland.

1994-12-30T15:00:00Z 森本, 佳成 http://hdl.handle.net/10564/1612 2017-06-11T23:20:26Z 1994-12-30T15:00:00Z

タイトル: 口腔領域における交感，感覚および運動神経支配に関する研究 ： Ⅰ. 顎関節，阻噂筋における神経支配 著者: 森本, 佳成 抄録: Sympathetic，sensory and motor innervation of the temporomandibular joint (TMJ) and the masticatory muscles was investigated in the rat by use of horseradish peroxidase (HRP) method. In the case of HRP application into TMJ，labeled cells were distributed uniformly in the superior cervical ganglion (SCG). In the trigeminal ganglion (TRG) they were found in the submandibular division. In TRG the mean diameter of labeled cells of TMJ (23.7±5.9μm) was significantly smaller than that of tooth pulp (27.4±5.0μm). Almost no labeling was obtained in SCG after sectioning of the cervical sympathetic plexus. By sectioning of the auriculotemporal nerve，labeling of TMJ was eliminated in SCG and reduced by 1/10 in TRG. The above observation indicated that the sympathetic components which originate from SCG innervate TMJ through intervention of the auricurotemporal nerve after ascending along the external carotic plexus ; also，that approximately 90％ of the sensory fibers innervating TMJ are carried by the auricurotemporal nerve，which seems to contain smaller Aδ and C fibers. HRP injection into the masseteric，temporal，medial pterygoid and lateral pterygoid muscles brought ipsilateral numerous labeled cells which localized at the central 1/2 of SCG，but only few cells in the anterior digastric and mylohyoid muscles. Labeled cells were found ipsilaterally in the submandibular division in TRG. The mean diameter of TRG labeled cells in the above muscles (24.3±4.9μm) was significantly smaller than that in tooth pulp. In the trigeminal motor nucleus，labeled cells after HRP injection into the masticatory muscles were localized in each rostro-caudal level. The distribution pattern of neuron size of the masseteric muscles was bimodal，and that of the others unimodal. The mean diameter of labeled cells of the masseteric (26.0±4.3μm) and temporal muscles (26.1±4.3μm) was significantly larger than that of the others.

1994-12-30T15:00:00Z 中島, 靖夫 http://hdl.handle.net/10564/1608 2017-05-29T06:09:29Z 1994-12-30T15:00:00Z

タイトル: 糖尿病性腎症における尿中糖蛋白分析 ： ウエスタンブロッティング分析によるレクチン結合パターン 著者: 中島, 靖夫 抄録: The aim of this study was to evaluate the clinical usefulness of polyacrylamide gel electrophoresis，followed by reaction with peroxidase-coupled lectins using urinary glycoproteins in 20 patients with diabetic nephropathy. After SDS-polyacrylamide gel electrophoresis (SDS-PAGE)，high performance lectin affinity chromatography (HPLAC) and immunoblotting of urinary glycoproteins with α₁-acid glycoprotein (α₁-AG) and α₁-microglobulin (α₁-MG) were performed. The lectins used were derived from Triticum vulgaris (WGA)，Phaseolus vulgaris (PHA-E₄)，Dolichos biflorus (DBA) and Lens culinaris (LCA)，which had a high affinity for β1→4 N-acetyl-D-gulcosamine (GlcNAcβ1→4GlcNAc)，N-acetyl-D-galactosamine (GalNAc)，α-galactosamine (α-GalNAc) and α-mannose (α-Man) residues，respectively. Electrophoretic patterns of urinary glycoproteins clearly showed the presence of lectin-reactive glycoproteins with molecular weights lower than that of albumin. The molecular weights of the main bands that reacted with WGA，namely PHA-E₄ and LCA，were respectively 50,000 and 38,000 daltons，and increased with the progression of diabetic nephropathy. WGA reacted strongly with many glycoproteins having a wide range of molecular weights. LCA and PHA-E₄ reacted preferentially with glycoproteins of molecular weight lower than 50,000 daltons，but with DBA no reaction was observed. These results suggest that low molecular-weight glycoproteins that are observed in the urine of patients with diabetic nephropathy have abundant carbohydrate residues such as GlcNAc β1→4 GlcNAc，GalNAc and α-Man，and include α₁-AG and α₁-MG. The excretion of low-molecular-weight glycoproteins with high affinities for some lectins suggests organic impairment in diabetic nephropathy.

1994-12-30T15:00:00Z