1996-04 http://hdl.handle.net/10564/1498 2026-04-10T15:40:47Z 2026-04-10T15:40:47Z 三馬, 省二 平尾, 佳彦 坂, 宗久 百瀬, 均 吉田, 克法 大園, 誠一郎 岡島, 英五郎 http://hdl.handle.net/10564/717 2017-05-29T06:07:10Z 1996-04-29T15:00:00Z

タイトル: 腹腔鏡下副腎摘除術 : 術式導入期5例の経験 著者: 三馬, 省二; 平尾, 佳彦; 坂, 宗久; 百瀬, 均; 吉田, 克法; 大園, 誠一郎; 岡島, 英五郎 抄録: From January 1993 to May 1994, 5 patients with unilateral adrenal tumor underwent laparoscopic adrenalectomy at the Department of Urology, Nara Medical University. The preoperative diagnoses were primary aldosteronism in 3 cases and nonfun- ctioning tumor in 2. Three were on the right side and 2 on the left side. The adrenal gland was removed laparoscopically in 4 patients ; one case underwent open hemostasis and extirpation because of intraoperative massive arterial bleeding caused by the slipping out of a hemoclip. In the 4 cases in which laparoscopic adrenalectomy was completed, the mean operative time was 401 minutes, ranging from 330 to 490 minutes, and estimated bleeding volume ranged from 25 to 288 ml (mean 131 ml). Operative complications were localized pulmonary embolism in one patient, localized renal infarction in one and subcutaneous emphysema in 2, which could be treated conservatively. Laparoscopic adrenalectomy requires practice and skill. However, it is less invasive, compared to open adrenalectomy. It is considered that laparoscopic surgery would be highly useful in the field of adrenal surgery.

1996-04-29T15:00:00Z 増井, 一弘 栗山, 茂樹 松本, 昌美 美登路, 昭 宮本, 洋二 阪本, たけみ 松田, 隆昭 山本, 浩治 城井, 啓 山根, 佳子 小林, 洋三 中谷, 敏也 奥, 和美 小泉, 雅紀 吉川, 正英 菊池, 英亮 松村, 雅彦 福井, 博 http://hdl.handle.net/10564/716 2017-05-29T06:06:28Z 1996-04-29T15:00:00Z

タイトル: 胃および十二指腸ポリープに対する留置スネアを用いた内視鏡的ポリペクトミーの経験 著者: 増井, 一弘; 栗山, 茂樹; 松本, 昌美; 美登路, 昭; 宮本, 洋二; 阪本, たけみ; 松田, 隆昭; 山本, 浩治; 城井, 啓; 山根, 佳子; 小林, 洋三; 中谷, 敏也; 奥, 和美; 小泉, 雅紀; 吉川, 正英; 菊池, 英亮; 松村, 雅彦; 福井, 博 抄録: Remarkable developments have been made recently in endoscopic ther- apies, such as endoscopic sphincterotomy, endoscopic injection sclerotherapy and endos- copic hematostatic procedures. Specifically, endoscopic polypectomy has been widely used for the resection of polyps in the digestive tract. As endoscopic polypectomy is employed for the excision of large polyps, the incidence of serious complications such as hemorrhage and perforation has been increasing. Hemorrhage is the most frequent serious complication in endoscopic polypectomy. Therefore, several procedures, such as local injection of hypertonic saline epinephrine, clipping and ligation methods have been devised to prevent the complication. We report here our experience of endoscopic polypectomy againt gastric and duodenal polyps by the use of snare loops. This method is easy to apply. We ligated the bottom of a polyp by a snare loop, and excised with an electrocautery snare. Case 1 had a relatively large-sized hyperplastic polyp, 30 mm in diameter, in the posterior wall of gastric antrum. Endoscopic ultrasonography revealed that the polyp had a vessel at its bottom. Cases 2 and 3 had adenomatous polyps in the duodenum of 10 mm and 70 mm in diameter, respectively. In all the cases, after ligating the bottom of a polyp by a snare loop, endoscopic polypectomy was carried out successfully at the proximal peripheral side with an electric coagulator without any complications. Endoscopic examination one week after the polypectomy revealed that there was a snare loop in case 1, which was removed by biopsy forceps without any bleeding, and that there remained no snare loops in cases 2 and 3. Thus it was demonstrated that ligation by a snare loop is a useful method for preventing hemorrhage in the endoscopic polypectomy of relatively large-sized gatroduodenal polyps.

1996-04-29T15:00:00Z Hatoko, Mitsuo Tada, Hideyuki Kuwahara, Masamitsu Muramatsu, Tsutomu Shirai, Toshihiko http://hdl.handle.net/10564/715 2017-06-11T23:20:26Z 1996-04-29T15:00:00Z

タイトル: EPINEPHRINE INDUCES 72-kD HEAT SHOCK PROTEIN (HSP72) IN CULTURED HUMAN FIBROBLASTS 著者: Hatoko, Mitsuo; Tada, Hideyuki; Kuwahara, Masamitsu; Muramatsu, Tsutomu; Shirai, Toshihiko 抄録: In order to know the stress response of cells to epinephrine, we examined the induction of 72-kD protein (HSP 72) in cultured human fibroblasts using an immuno- fluorescence method. HSP 72 was induced strongly and rapidly after epinephrine treatment at a final concentration of 2.7×10⁻⁵ M which corresponds to the concentration of clinical use. When propranolol (1×10⁻⁴ M) was added to a culture medium containing epinephrine, the onset and peak of HSP 72 induction of fibroblasts were delayed compared to those observed after epinephrine treatment alone. These results suggest that epinephrine is actually a potent inducer of HSP 72 and that the cells are stressed by epinephrine at a concentration of routine use in surgery. Furthermore, the induction of HSP 72 by epinephrine is considered to be related to its β-adrenergic actlon.

1996-04-29T15:00:00Z 増井, 一弘 http://hdl.handle.net/10564/714 2017-05-29T06:06:28Z 1996-04-29T15:00:00Z

タイトル: 細菌由来シトシンデアミナーゼ遺伝子と5-フルオロシトシンによる癌に対する遺伝子治療に関する基礎的研究 著者: 増井, 一弘 抄録: The gene for bacterial cytosine deaminase (CD) which metabolites the innocuous prodrug 5-fluorocytosine (5-FC) to the highly toxic drug 5-fluorouracil (5-FU) was transduced via retrovirus into several cell lines : hepatocllular carcinoma(HCC), squamous cell carcinoma (SCC) and myoblastoma. Transduction of the CD gene did not affect the growth rate of these cells. However, the cells genetically modifibd to express CD were sensitive to 5-FC in a dose-dependent manner, and the modified HCC, SCC and myoblastoma cells exhibited approximately 120-, 130- and 460-fold higher sensitivity to 5 -FC, respectively, compared with each of the corresponding parental cells. Cells expressing CD were killed with 5-FC at concentrations achievable in sera of patients receiving clinical doses of 5-FC. Conversely, there were no significant differences in the susceptibility to 5 -FU between the modified cells and their parental cells. The cells expressing CD were able to induce the killing of their neighboring parental cells in the presence of 5-FC not only when they were in contact with each other but also when they were not. The killing ability of cells expressing CD was closely correlated with amounts of 5-FU generated by the cells. Most importantly, this killing effect was also observed in vivo. Significant antitumor effects were induced in mice bearing HCC cells composed of the modified and unmodified cells by an intraperitoneal injection of 5-FC. When genetically modified HCC cells com- prised only 10% of a tumormass, significant suppression of HCC development was achieved by administration of subtoxic doses of 5-FC. These results demonstrated the feasibility of gene therapy using the bacterial CD gene and 5-FC for the treatment of cancers.

1996-04-29T15:00:00Z